qPCR for Gene Therapies with the Cell and Gene Therapy Catapult

The Synthace platform provides the flexibility required for this type of work. Instead of manually dictating every step, the platform is able to make intelligent decisions to suggest the most efficient workflows which are easily adaptable when needed. Georgie Hermitage, PhD, Analytical Development Scientist The Cell and Gene Therapy Catapult

Scaling up qPCR with a flexible & intuitive software platform

The Cell and Gene Therapy Catapult began working with Synthace in 2018, when they realized the need to automate their routine analytical assays that require repetitive and complex manual actions.

They utilized Synthace’s software platform to perform scalable and flexible qPCR assays on automated liquid handlers through a user-friendly interface that is intuitive to biologists.

Key findings

By utilizing Synthace to automate routine qPCR assays, the Cell and Gene Therapy Catapult achieved:

Scalable

384-well plate assays

Assays could be easily scaled up and down to required numbers in seconds

5 hour

saving in hands-on time

Only 30 minutes of manual input from sample preparation to data generation

90-110%

qPCR efficiency

Assays met the strict quality criteria and maintained data integrity

Synthace provides a virtual preview of the experiment to be executed. The preview window enables users to verify the experimental details in a matter of minutes and, if needed, modify their workflow prior to execution without having to perform multiple trial-and-error runs in the lab wasting precious samples, reagents, and time.

 

 

Whether executed manually or with Synthace, qPCR included ~30 minutes of manual sample preparation with 1 hour of assay run time in a thermocycler (walkaway time). However, manual assays also included up to 5 hours of additional hands-on time needed to prepare a full 384-well plate. Synthace did this automatically in 1 hour 45 minutes, all of which was walkaway time for scientists. Overall, Synthace reduced hands-on time by 5 hours, with only ~30 minutes of manual input required from initial sample preparation to data generation.

 

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