Addressing DOE and Data Challenges in Downstream Bioprocessing

Synthace at BioProcess International US West 2021

Watch our presentation and panel discussion to learn more about DOE and data challenges in miniaturized purification workflows

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Meet our bioprocessing experts

Jordan Sharpened
Jordan Ang, PhD

Senior Scientist and Bioinformatician

On Monday 29 March 2021 11:55am - 12:15pm (PST/PDT) Jordan presented his work on purification process development using DOE: Characterizing transaminase purification using a space-fill DOE.

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Luke Conference Crop
Luke Cach

Scientific Solutions Lead

2:05pm - 2:35pm (PST/PDT) Luke joined scientists from Biogen, Sanofi and KBI Biopharma to talk about the importance of data and experimental connectivity in purification development.

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  • Bioprocess informatics
  • Bioreactor integrations
  • Connecting upstream and downstream PD
  • Knowledge transfer within and across teams
Jack Conference
Jack Kiely

Associate Scientific Consultant

Jack was on call throughout the conference to answer questions on automation or our software Antha.j

Didn't get a chance to connect? Reach out to us anytime at demo@synthace.com
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Introducing Antha®

The world’s most powerful software platform for performing biological experiments

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Watch our presentation and panel discussion to learn more about DOE and data challenges in miniaturized purification workflows

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Presentation by Dr Jordan Ang: Characterizing Transaminase Purification Using a Space-Fill DOE

Aided by a fully automated and fully traceable, end-to-end workflow for miniaturized protein purification, we performed a space-fill Design of Experiments (DOE), designed in JMP, to characterize the purification of recombinant transaminase protein from E. coli cell lysate, via gradient anion exchange chromatography.

Jordan presents our methods and results from a total of 48 RoboColumns® (run over two eight-hour sessions), involving six different resin types and varying combinations of sample and buffer salt concentrations, buffer pHs, and chromatography flow rates. Measurement data was automatically structured and analysed for both yield (from A280 absorbance) and purity (using LabChip® GXII Touch™).

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Panel Discussion with Synthace's Luke Cach and experts from Biogen, Sanofi and KBI Biopharma: The Importance of Data and Experimental Connectivity for Rapid Purification Development

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See Antha in action in our recorded online demos

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