What Makes Synthace a Game Changer for Automated Miniaturised Purification?

Repligen’s OPUS® RoboColumns® have been the industry standard in enabling automated miniaturised purification since 2007. Now sold under a variety of different brands, their pre-packed design enables process development scientists to perform multiplexed purification experiments via Tecan’s automated Te-Chrom™ system.

Optimising Automated Miniaturised Purification

By efficiently exploring the purification design space, process development scientists can optimise chromatography capture and yield of their purified protein of interest. This can be achieved by investigating the many factors that impact the purification process. Factors like buffer composition, pH and residence time, for example.

Critical to this optimisation is understanding how multiple factors work in combination to impact the purification outcomes. To achieve this level of understanding, a multifactorial, statistical approach known as Design of Experiments (DoE) needs to be taken.

The insight provided by a sophisticated DoE enables an iterative, experimental approach that can drastically improve the efficiency of the purification process. However, translating sophisticated DoE plans into instructions for an automated purification experiment is not often straightforward or flexible in existing software. Programming the liquid handling steps necessary to carry out a DoE with a high number of factors can be a daunting task even for experienced automation engineers.

Data Management and Alignment for Batch Users

When running workflows using miniaturised columns it is often cumbersome to keep track of the many data files generated, and how these correspond with the different chromatography stages.  In particular, various data corrections, like path length correction or A340 based background subtraction, are often surprisingly cumbersome to apply to robocolumn based workflows. The Synthace platform automatically applies such data-normalising corrections and aligns plate reader data with the sample liquid handling steps and chromatography phases, leading to quicker insight into your process. Pseudo Chromatograms are automatically generated using the Synthace protocol for miniaturised purification. And, concentration and yield are automatically calculated as well.

The Synthace platform also conveniently keeps track of the details associated with your purification run: instrument configuration as well as sample and buffer metadata are stored in the cloud, as are time of execution and operator information, all with a unique identifier. This allows solid traceability among the many runs you may be doing in batch processing runs where one would vary growth conditions in a bioreactor, for example.

Finally, the Synthace platform's purification protocol ties into the grander vision of bioprocessing informatics, where it also enables alignment with external data, e.g. from bioreactors like the Ambr250 or downstream processes like ELISA or electrophoresis.

Listen to our Senior Automation Engineer, Arne Vandenbroucke’s explanation:

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