Automated, High-Throughput IgG Quantification with Synthace and Valita®TITER

Key findings
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  • By combining our software platform Synthace and the Valita®TITER assay, scientists can now simply and efficiently automate an off-the-shelf IgG quantification assay to speed up their cell line development pipelines
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  • Synthace reduced the total hands-on time by 55%, with a 61% saving in planning time and 53% saving in assay execution time
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  • Synthace-driven automation achieved comparable performance to manual execution, with the average interplate standard deviation of 0.84 mP for manual runs and 1.66 mP for automated runs for different IgG concentrations
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Valita®TITER and Synthace

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Valita®TITER, developed by Valitacell, is a simple plate-based assay for the quantification of Fc-containing IgG-derived monoclonal antibodies in cell culture supernatant and media, using fluorescence polarization (FP) for detection.
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The assay is applicable to all stages of the biologic’s development pipeline, from initial discovery and clonal characterization, all the way to process optimization and production monitoring.
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Synthace is a cloud-based software platform that empowers scientists to design and execute complex automated experiments in a hardware-agnostic fashion and without any prior programming experience. When executing assays amenable to automation, such as Valita®TITER, Synthace is able to flexibly and efficiently perform dilution calculations, prepare plates, and change assay parameters.

 

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