Automated qPCR

Why qPCR with Synthace?

qPCR has become a mainstay of molecular biology. As the need for higher throughput and reproducibility grows many labs have begun to automate their qPCR process, requiring more complex automation protocols and more advanced users. 

Synthace allows for automated, robust, and rapid qPCR experiment design

This removes the need for advanced programming, allowing more access to automation.

Automate

Synthace automates qPCR workflows for reproducible experiments and more walk-away time

Optimize

Synthace’s optimized liquid handling policies are proven to give smaller variance in pipetting volumes

Trace

Synthace integrates with qPCR machines offering full sample provenance

Pain points and current processes

  • Current practices

    With Synthace

  • Experiment inputs

    Current practices

    • Manual qPCR experiments can be time-consuming and error-prone
    • Difficult automation programming makes qPCR setup tedious

    With Synthace

    • Automated qPCR workflows create all liquid handling steps based on your qPCR design
    • User friendly interface allows for easy experimental design for more approachability
  • Experiment planning

    Current practices

    • qPCR experiments require precise intermediate dilution calculations
    • Leads to more time planning and smaller experiments

    With Synthace

    • Plan your experiment remotely, from anywhere
    • Flexible programming makes changing experimental parameters or design seamless
    • Synthace automatically performs all liquid calculations leaving more time to plan additional experiments
  • Experiment execution

    Current practices

    • User needs to understand liquid handling hardware details to modify execution
    • Integration of multiple devices can be unreliable or difficult to manage

    With Synthace

    • Synthace is device-agnostic, enabling protocol execution on multiple liquid handling devices and qPCR machines
    • Synthace’s library of optimized liquid handling policies reduces volume transfer errors and cross-contamination
  • Experimental traceability

    Current practices

    • Transferring data from a qPCR machine for analysis can be cumbersome
    • No simple way to structure sample metadata with qPCR data

    With Synthace

    • Synthace provides full sample traceability from input to analysis
  • Experiment outputs

    Current practices

    • Difficult to compare data and methods with previous runs
    • Sharing experimental protocols between lab members and devices can be challenging

    With Synthace

    • Previous data sets stored in the cloud for easy access
    • Share flexible workflows between team members at different sites and between different automation devices

Protocol walkthrough

Basic concepts

Translate human-readable actions through machine instructions

Simple building blocks...

Elements are a key concept in Synthace. They translate human readable actions into machine instructions. You can define how the experiment will be performed through the element input parameters.

Elements form the visual user interface to Synthace’s planning engine. Synthace interprets your requirements and translates these into complex robot instructions. Input volumes are also calculated and the labware needed is determined.

...translated into complex robot instructions

You can create workflows in Synthace by connecting elements together to specify the flow of physical materials and information.

A simple workflow example is connecting an element where liquids and their properties are defined (e.g. liquid names, concentrations, pH, and so on) with an element that aliquots these liquids onto a plate as desired.

Define experiment

Easily define and modify your experiment

Easily upload your qPCR design

Synthace converts your qPCR design into instructions for any device you are using

Quickly view entire experiment in silico

See how Synthace calculates all liquid transfer steps in order to give the desired mastermix concentrations

Schedule your workflow to any of your devices

Synthace is device-agnostic, connecting to various liquid handlers and qPCR machines

Preview in silico

Preview your runs in detail at your desk

Synthace’s execution preview shows a layout of your device, and clearly shows where labware needs to be placed and the amount and location of your reagents.

The preview also shows every liquid handling action in order of execution with a useful step-by-step time estimate. This minimizes the chances of errors, and gives you an immediate estimate of walk-away time. Hovering over individual transfers will indicate the selected liquid handling policies.

Output

Data structuring and export

Once the experiment has completed, the user's data is securely sent back to the Synthace cloud. This data can then be exported in structured data tables in common formats. All data and methods can easily be compared with previous runs.

Introducing Synthace, the world’s most powerful software platform for performing biological experiments

​​Intelligent software for the dynamic use of laboratory automation

Sophisticated experiments made simple

FAQs

Synthace for qPCR is available on:

  • Tecan's Freedom EVO®
  • Hamilton's Microlab® STAR and STARlet
  • Gilson's PIPETMAX®
  • FORMULATRIX®'s MANTIS®

Synthace for qPCR does not support analytical methods at this time, but it may provide a setup file for the QuantStudio™ 7 qPCR instrument. If data structuring and analysis are required, please contact us for further information by sending us an email or booking a custom demo at the top of the page.

The Synthace planning engine parses the high-level inputs specified in your workflow, including the experimental design, selected labware and deck layout. The planning engine converts these instructions into low-level instructions such as the liquid handling steps and the exact labware positions on your predefined deck layout.

Yes, custom deck layouts can be chosen in Synthace. The user can define multiple deck configurations for the same liquid handler mode.

SynthaceHub automatically sends log and configuration files to the cloud for storage in your organization’s private data repository in Synthace from the liquid handler’s native software.

Although calendar integrations are not yet available in Synthace, the estimated run times as calculated by Synthace’s planning engine provide an accurate timing for you to ensure that you have booked enough time on your desired devices to execute your experiments.

Synthace will work out the changes necessary to implement your workflow. You simply need to upload your deck configuration and re-simulate — Synthace will use your new worktable layout to determine the necessary instructions.

Synthace comes with its own library of different liquid handling policies for all types of liquids. You can select different liquid handling policies for each of the liquid handling steps. Different samples can have different policies, and these policies can be linked to your liquid classes on certain liquid handling devices.

Yes, Synthace’s cherry picker is one example of that. Another example would be DOE runs with hard to change factors where we would need to run across two workflows.

Experimental results can be searched and accessed by everyone in your group/organization who has an account in Synthace. If you would like to discuss access control and data security in more detail, please drop us an email or use the “Request a demo” button at the top of the page.

Trusted by

We were founded by a team of biologists in 2011 as a bio-process service provider, optimizing the existing bio-processes of our clients using a multi-factorial experimental approach. We used automated robotic equipment to run our high-dimensional, complex experiments.

Synthace has helped us improve our business and think outside the box, and its platform has allowed us to do many things we previously couldn’t” André Raposo Group Lead - Computer-Aided Biology (CAB) group at Oxford Biomedica
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