Flexibly plan your DOE media optimization workflow
Upload your design parameters in Synthace’s workflow builder.
Media optimization is a necessary part of optimized cell growth. With so many factors playing a part, traditional one-factor-at-a-time (OFAT) methods can require a multitude of optimization tests, costing time and resources.
Design of Experiments (DOE) cuts down on experimental runs by testing multiple factors and their interactions simultaneously. Synthace translates multi-factorial experimental designs into executable steps for your automation devices, allowing for complex experiments and removing the need for expert users.
Synthace makes translating your DOE design into actual instructions for your device easy
Synthace’s methods and runs are fully traceable for easy review and comparison
Synthace merges data acquisition and structuring from analytical devices for faster analysis
Elements are a key concept of the Synthace platform. They translate human readable actions into machine instructions. You can define how the experiment will be performed through the element input parameters.
Elements form the visual user interface to Synthace’s planning engine. Synthace interprets your requirements and translates these into complex robot instructions. Input volumes are also calculated and the labware needed is determined.
You can create workflows in Synthace by connecting elements together to specify the flow of physical materials and information.
A simple workflow example is connecting an element where liquids and their properties are defined (e.g liquid names, concentrations, pH, and so on) with an element that aliquots these liquids onto a plate as desired.
Flexibly plan your DOE media optimization workflow
Upload your design parameters in Synthace’s workflow builder.
Review all experimental steps in silico
Synthace converts your workflow into readable steps for all your devices, automatically creating all necessary intermediate liquids.
All files in one place
Exported experimental data, plate files, device instructions and reagents required can be found in Synthace’s execution details page, saving time to plan future experiments.
Synthace’s execution preview shows a layout of your device, and clearly shows where labware needs to be placed and the amount and location of your reagents.
The preview also shows every liquid handling action in order of execution with a useful step-by-step time estimate. This minimizes the chances of errors, and gives you an immediate estimate of walk-away time. Hovering over individual transfers will indicate the selected liquid handling policies.
Once the experiment has completed, the user’s data is securely sent back to Synthace. Plate reader data is aligned with the sample liquid handling steps, and normalized for pathlength and blank corrected where appropriate.
Synthace for DOE: Media Optimization is available on:
Synthace provides a wide range of integration options for analytical instruments. We have direct integration with most multimode plate readers commonly used. If you’d like to enquire about specific processes for your lab, please drop us an email or use the “Request a demo” button above.
The Synthace planning engine parses the high-level inputs specified in your workflow, including the experimental design, selected labware and deck layout. The planning engine converts these instructions into low-level instructions such as the liquid handling steps and the exact labware positions on your predefined deck layout.
Yes, custom deck layouts can be chosen in Synthace. The user can define multiple deck configurations for the same liquid handler mode.
SynthaceHub automatically sends log and configuration files to the cloud for storage in your organization’s private data repository in Synthace from the liquid handler’s native software.
Although calendar integrations are not yet available in Synthace, the estimated run times as calculated by Synthace’s planning engine provide an accurate timing for you to ensure that you have booked enough time on your desired devices to execute your experiments.
Synthace will work out the changes necessary to implement your workflow. You simply need to upload your deck configuration and re-simulate — Synthace will use your new worktable layout to determine the necessary instructions.
Synthace comes with its own library of different liquid handling policies for all types of liquids. You can select different liquid handling policies for each of the liquid handling steps. Different samples can have different policies, and these policies can be linked to your liquid classes on certain liquid handling devices.
Yes, Synthace’s cherry picker is one example of that. Another example would be DOE runs with hard to change factors where we would need to run across two workflows.
Experimental results can be searched and accessed by everyone in your group/organization who has an account in Synthace. If you would like to discuss access control and data security in more detail, please drop us an email or use the “Request a demo” button above.
We were founded by a team of biologists in 2011 as a bio-process service provider, optimizing the existing bio-processes of our clients using a multi-factorial experimental approach. We used automated robotic equipment to run our high-dimensional, complex experiments.
Synthace has helped us improve our business and think outside the box, and its platform has allowed us to do many things we previously couldn’t”André Raposo Group Lead - Computer-Aided Biology (CAB) group at Oxford Biomedica