---
title: Optimizing Construct Assembly with Synthace - Synthace
description: Synthace improved robustness of BsaI DNA-assembly resulting in >95% correct constructs, achieved a 74% time saving, and reduced tip use by 22%.
image: https://www.synthace.com/hubfs/Imported_Blog_Media/BacteriaPetriDish-e1596361615776-1.png
---

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July 21, 2019

# Optimizing Construct Assembly with Synthace

Written by: [Synthace Team](https://www.synthace.com/blog/author/synthace-team)

## Key findings

- Synthace improved the robustness and reproducibility of Design of Experiments (DOE) optimization of BsaI DNA-assembly protocols, ensuring **>95% correct 7-part DNA assemblies  
  **
- The optimized experimental workflow was initially programmed for a Gilson PIPETMAX® liquid handler and easily transferred to a Hamilton Microlab® STAR, FORMULATRIX® MANTIS®, and Tecan Freedom EVO® robots, achieving comparable robustness
- The automated Synthace approach reduced time spent per experiment from 29.5 hours to 8.5 hours, resulting in a 21-hour time saving; **up to 63 hours were saved** across the whole study
- Overall, Synthace achieved a **74% time-saving** in experimental planning, design, and execution. This means large efficiency savings in FTE time and a **22% reduction in tip usage** 

![](https://www.synthace.com/hubfs/Imported_Blog_Media/efficiencies-e1627765277736-768x957-1.png)

## The Proof is in the Results

Discover how Synthace-driven DOE optimization of 7-part DNA assembly achieved:

- >95% correct constructs
- 74% time-saving
- 22% reduction in tip usage

[ Read the full report ](https://synthace.showpad.com/share/ERsHblfqn8qDhTulBB8zD)

Tag(s): [Applications](https://www.synthace.com/blog/tag/applications)

## [Synthace Team](https://www.synthace.com/blog/author/synthace-team)

## Other posts you might be interested in

[View All Posts](https://www.synthace.com/blog)

[

Lab of the Future

## Dynamic Binding Capacity with Synthace and Tecan Te-Chrom™

Synthace was leveraged to assess the Dynamic Binding Capacity (DBC) of two different resins, using miniaturized columns and the Tecan Te-Chrom™ system.

![Synthace Team](https://www.synthace.com/hubfs/2022-new-website/Blog%20Assets/Synthace-Brandmark-White.png) 

 Synthace Team

** 5 min 

](https://www.synthace.com/blog/dynamic-binding-capacity-with-synthace-and-tecan-te-chrom) [

People

## Engineering Biology with Synthace

When I was 17 I thought I had to choose one subject and devote my future career to a single passion... but I had a multidisciplinary future ahead of me!

![Nuno Leitão, PhD](https://www.synthace.com/hubfs/Screenshot%202022-10-06%20at%2015.36.44.png) 

 Nuno Leitão, PhD

** 3 min 

](https://www.synthace.com/blog/engineering-biology-with-synthace) [

Platform updates

## Synthace just got a whole lot better: 4 improvements biologists need to hear about

Last month we released our biggest update of Synthace so far. This article explains why that's important news for biologists

![Synthace Team](https://www.synthace.com/hubfs/2022-new-website/Blog%20Assets/Synthace-Brandmark-White.png) 

 Synthace Team

** 11 min 

](https://www.synthace.com/blog/synthace-just-got-a-whole-lot-better-4-improvements-biologists-need-to-hear-about)

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