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    July 21, 2019

    Optimizing Construct Assembly with Synthace

    Key findings

    • Synthace improved the robustness and reproducibility of Design of Experiments (DOE) optimization of BsaI DNA-assembly protocols, ensuring >95% correct 7-part DNA assemblies
    • The optimized experimental workflow was initially programmed for a Gilson PIPETMAX® liquid handler and easily transferred to a Hamilton Microlab® STAR, FORMULATRIX® MANTIS®, and Tecan Freedom EVO® robots, achieving comparable robustness
    • The automated Synthace approach reduced time spent per experiment from 29.5 hours to 8.5 hours, resulting in a 21-hour time saving; up to 63 hours were saved across the whole study
    • Overall, Synthace achieved a 74% time-saving in experimental planning, design, and execution. This means large efficiency savings in FTE time and a 22% reduction in tip usage 

    The Proof is in the Results

    Discover how Synthace-driven DOE optimization of 7-part DNA assembly achieved:

    • >95% correct constructs
    • 74% time-saving

    • 22% reduction in tip usage

    Tag(s): Applications

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