---
title: Dynamic Binding Capacity with Synthace and Tecan Te-Chrom™ - Synthace
description: Synthace was leveraged to assess the Dynamic Binding Capacity (DBC) of two different resins, using miniaturized columns and the Tecan Te-Chrom™ system.
image: https://www.synthace.com/hubfs/Imported_Blog_Media/website-banner-1.png
---

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November 9, 2020

# Dynamic Binding Capacity with Synthace and Tecan Te-Chrom™

Written by: [Synthace Team](https://www.synthace.com/blog/author/synthace-team)

## Study summary

- Using Synthace, we evaluated the Dynamic Binding Capacity (DBC) of two different resins packed in 200 µL RoboColumns® on a Tecan Freedom EVO® liquid handler, at different residence times
- Synthace structured A280 nm absorbance data from the plate reader, automatically created a pseudo-chromatogram and normalized the data by applying pathlength and blank correction.
- Using Synthace's data download capability, the normalized data was subsequently imported in Python to perform further smoothing and DBC analysis

 

![](https://www.synthace.com/hs-fs/hubfs/Imported_Blog_Media/Figure-6B-e1605692032459-768x276-1.png?width=1400&name=Figure-6B-e1605692032459-768x276-1.png)

 

## Key findings

- MabSelect™ PrismA and Praesto® Jetted A50 resins have comparable DBC at shorter residence times
- Synthace enables flexible programming of RoboColumn® workflows, allowing users to specify residence times and fractions collected per individual column. Synthace workflows can be shared between teams to facilitate knowledge transfer and reduce experimental design time...
- Synthace promotes higher quality data by using unique identifiers for each experiment, structuring data in common formats, and keeping track of experimental metadata  
  ...

![](https://www.synthace.com/hs-fs/hubfs/Imported_Blog_Media/Figure-8-e1605692060722-768x359-1.png?width=1400&name=Figure-8-e1605692060722-768x359-1.png)

### [Read the full report here](https://synthace.showpad.com/share/RdCnUltXM5xtfI3AveVGK)

Tag(s): [Lab of the Future](https://www.synthace.com/blog/tag/lab-of-the-future) , [Applications](https://www.synthace.com/blog/tag/applications)

## [Synthace Team](https://www.synthace.com/blog/author/synthace-team)

## Other posts you might be interested in

[View All Posts](https://www.synthace.com/blog)

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## Optimizing Construct Assembly with Synthace

Synthace improved robustness of BsaI DNA-assembly resulting in >95% correct constructs, achieved a 74% time saving, and reduced tip use by 22%.

![Synthace Team](https://www.synthace.com/hubfs/2022-new-website/Blog%20Assets/Synthace-Brandmark-White.png) 

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Data

## Miniaturized Purification with Synthace and Tecan Te-Chrom™: Uniting Robust Execution With Flexible Planning and Comprehensive Data Analysis

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** 5 min 

](https://www.synthace.com/blog/miniaturised-purification-with-synthace-and-tecan-te-chrom-uniting-robust-execution-with-flexible-planning-and-comprehensive-data-analysis) [

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## Synthace Expert Interviews: Cell and Gene Therapies with Dr Damian Marshall

Synthace Expert Interviews: Cell and Gene Therapies with Dr Damian Marshall Filter

![Synthace Team](https://www.synthace.com/hubfs/2022-new-website/Blog%20Assets/Synthace-Brandmark-White.png) 

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** 9 min 

](https://www.synthace.com/blog/synthace-expert-interviews-cell-and-gene-therapies-with-dr-damian-marshall)

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