What is Design of Experiments?
Design of Experiments (DOE) is a holistic approach that allows you to study the effect of multiple factors and their interactions on an assay in parallel. With so many factors playing a part, traditional one-factor-at-a-time (OFAT) methods are no longer feasible as they can require a multitude of optimization tests, costing time and resources.
Rapid, automated execution of a high-dimensional space-filling Design of Experiments for assay development
In this case study, we combine the speed and accuracy of SPT Labtech’s dragonfly® discovery dispenser with the flexible planning and data aggregation capabilities of Synthace’s software platform to unlock the possibility of physically executing a 6-factor space-filling DOE to comprehensively characterize the design space for an enzymatic assay in a single rapid experiment.
Runs in space-fill DOE
Two sets of triplicates of 384 runs and corresponding controls
Liquid handling steps
Total number of pipetting steps needed to perform the space-fill DOE
The DOE campaign was executed in one work day by a single user
The use of Synthace reduced time spent on planning the 20,745 liquid handling steps and data aggregation from the microplate reader, by 75% and 94% respectively. The dragonfly® discovery reduced liquid handling time by 98% compared with an average pipetting robot, as well as eliminating 99.9% of the number of tips needed.