Automated ELISA

Why ELISA with Synthace?

ELISA is a widely used technique for assaying target proteins and measuring contamination in bioprocesses, such as host cell protein (HCP).

Synthace can power up your ELISA by enabling flexible automated setup, execution and data analysis of your protocols.

Synthace enables rapid ELISA experiment design and optimization. This removes the need for advanced programming, allowing scientists more access to automation.


Synthace makes planning for ELISA easier and more flexible with dynamic automation reprogramming


Synthace integrates data acquisition and structuring from analytical devices for faster analysis


Synthace methods and runs are fully traceable for easy review and comparison

Pain points and current processes

  • Current practices

    With Synthace

  • Experiment inputs

    Current practices

    • Can’t easily define sample dilutions and replicates
    • Can’t define complex antibody pairings

    With Synthace

    • Flexible sample setup allows the user to easily define samples, replicates, dilutions and standards
    • Undertake more complex experimental designs, with automatic pairing of antibodies and antigens
  • Experiment planning

    Current practices

    • Switching between ELISA protocol types is difficult
    • Editing steps within an ELISA protocol can be difficult

    With Synthace

    • Plan your experiment remotely, from anywhere
    • Flexibly change experimental parameters and protocols with Synthace’s intuitive interface and dynamically reprogram the automation
    • Easily change between different ELISA protocols, share flexible workflows with team members
  • Experiment execution

    Current practices

    • User needs to understand liquid handling hardware details to modify execution
    • Integration of multiple devices can be unreliable or difficult to manage

    With Synthace

    • Simply send the generated method to the automation device for execution through SynthaceHub
    • Synthace handles the execution of the experiment, providing more than just a worklist
    • Synthace manages the software integration between the liquid handler and peripheral devices
  • Data aggregation

    Current practices

    • Transferring data from plate reader for analysis can be cumbersome
    • No simple way to structure sample metadata with plate reader data

    With Synthace

    • Synthace enables full sample provenance. Plate reader data, sample metadata and antibody metadata are all tracked
    • Synthace auto-structures plate reader data with sample IDs
    • Data can be ingested from integrated and non-integrated plate readers
  • Data analysis

    Current practices

    • External software packages are often needed for data analysis, requiring additional data management steps
    • User has to manually identify standards, create regression curves and estimate concentrations

    With Synthace

    • Synthace auto-creates regression curves and generates concentration estimates for samples
    • No extra analysis software needed
  • Experiment outputs

    Current practices

    • Difficult to compare data and methods with previous runs
    • Sharing experimental protocols between lab members and devices can be challenging

    With Synthace

    • Previous data sets stored in the cloud for easy access
    • Share flexible workflows between team members at different sites and between different automation devices

Protocol walkthrough

Basic concepts

Translate human-readable actions into machine instructions

Simple building blocks...

Elements are a key concept in Synthace. They translate human readable actions into machine instructions. You can define how the experiment will be performed through the element input parameters.

Elements form the visual user interface to Synthace’s planning engine. Synthace interprets your requirements and translates these into complex robot instructions. Input volumes are also calculated and the labware needed is determined.

...translated into complex robot instructions

You can create workflows in Synthace by connecting elements together to specify the flow of physical materials and information.

A simple workflow example is connecting an element where liquids and their properties are defined (e.g. liquid names, concentrations, pH, and so on) with an element that aliquots these liquids onto a plate as desired.

Define experiment

Easily define and modify your experiment

Define the assay you wish to execute

Set out the assays you wish to execute. Easily define:

  • Serial dilutions of samples and standards
  • Numbers of replicates
  • Controls for your experiment

You can also pair samples to different plate coatings if required.

Set the parameters for your protocol

Edit your workflow easily with the modular elements that allow you to easily lay out the steps of the protocol. Change the parameters within each step as required. Users can alter parameters such as incubation times, transfer volumes, wash protocols and liquid handling policies.

Select multiple devices for execution

Select multiple devices to execute the entire workflow. Devices can either be fully accessible to the central liquid handler in a device group or function separately. Synthace will control plate movement to devices within a device group or provide the user with prompts to move plates to stand alone devices.

Preview in silico

Preview your runs in detail at your desk

Synthace’s execution preview shows everything you need to visualize your workflow prior to execution. See how Synthace calculates all liquid transfer steps in order to achieve the desired assay and organizes plate movement between devices within a multidevice workflow. Layouts of your device and plates are provided to enable setup in the lab.

The preview also shows every liquid handling action in order of execution with a useful step-by-step time estimate. This minimizes the chances of errors, and gives you an immediate estimate of walk-away time. Hovering over individual transfers will indicate the selected liquid handling policies.

Automated execution

Send to the lab for automatic execution

Schedule your experiment from your desk

Run the whole workflow across multiple devices from SynthaceHub

Data analysis

Acquire, structure and visualize your data in one easy step

Following acquisition, plate reader data is automatically uploaded to the cloud

Synthace auto-associates plate reader data with the assay plate layouts set up by the liquid handler

Interrogate your data using the visual interface or download for further analyses


Synthace for ELISA is available on Hamilton Microlab® STAR and STARlet® liquid handlers with BioTek® 405 washer and BMG, Tecan or BioTek plate readers.

Synthace provides a wide range of integration options for analytical instruments. We have direct integration with some of the most common multimode plate readers used for absorbance reads for ELISA. If you’d like to enquire about specific processes for your lab, please drop us an email or use the “Request a demo” button at the top of the page.

Integrated plate reader data is automatically fitted against standards using 4 parameter logistic or linear regression and can be visualized and reviewed within Synthace before exporting processed results.

The Synthace planning engine parses the high-level inputs specified in your workflow, including the experimental design, selected labware and deck layout. The planning engine converts these instructions into low-level instructions such as the liquid handling steps and the exact labware positions on your predefined deck layout.

Yes, custom deck layouts can be chosen in Synthace. The user can define multiple deck configurations for the same liquid handler mode.

SynthaceHub automatically sends log and configuration files to the cloud for storage in your organization’s private data repository in Synthace from the liquid handler’s native software.

An ELISA experiment can be executed with an integrated system with a liquid handler, plate washer and reader physically joined, or by executing the liquid handling and washing steps with a physically disconnected plate reader connecting the data. SynthaceHub will bring in the outcomes from pipetting with the plate reader for data processing in the cloud software.

Although calendar integrations are not yet available in Antha, the estimated run times as calculated by Synthace's planning engine provide an accurate timing for you to ensure that you have booked enough time on your desired devices to execute your experiments.

Synthace will work out the changes necessary to implement your workflow. You simply need to upload your deck configuration and re-simulate — Synthace will use your new worktable layout to determine the necessary instructions.

Antha comes with its own library of different liquid handling policies for all types of liquids. You can select different liquid handling policies for each of the liquid handling steps. Different samples can have different policies, and these policies can be linked to your liquid classes on certain liquid handling devices.

Yes, Synthace’s cherry picker is one example of that. Another example would be DOE runs with hard to change factors where we would need to run across two workflows.

Experimental results can be searched and accessed by everyone in your group/organization who has an account in Synthace. If you would like to discuss access control and data security in more detail, please drop us an email or use the “Request a demo” button at the top of the page.

Trusted by

Synthace has helped us improve our business and think outside the box, and its platform has allowed us to do many things we previously couldn’t” André Raposo Group Lead - Computer-Aided Biology (CAB) group at Oxford Biomedica