Dynamic Binding Capacity with Synthace and Tecan Te-Chrom™

Study summary
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  • Using Synthace, we evaluated the Dynamic Binding Capacity (DBC) of two different resins packed in 200 µL RoboColumns® on a Tecan Freedom EVO® liquid handler, at different residence times
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  • Synthace structured A280 nm absorbance data from the plate reader, automatically created a pseudo-chromatogram, and normalized the data by applying pathlength and blank correction
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  • Using Synthace's data download capability, the normalized data was subsequently imported in Python to perform further smoothing and DBC analysis

Key findings
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  • MabSelect™ PrismA and Praesto® Jetted A50 resins have comparable DBC at shorter residence times
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  • Synthace enables flexible programming of RoboColumn® workflows, allowing users to specify residence times and fractions collected per individual column. Synthace workflows can be shared between teams to facilitate knowledge transfer and reduce experimental design time...
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  • Synthace adheres to the FAIR principles by using unique identifiers for each experiment, structuring data in common formats, and keeping track of experimental metadata
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