Synthace-Driven ELISA

Key findings
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  • Synthace performs end-to-end automated ELISAs, driving experiments from design to data analysis in a single platform
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  • Synthace links three independent devices into a responsive workcell for ELISA, shares status updates, and provides immediate feedback to the user through their personal computer
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  • Synthace achieves 71% reduction in total hands-on time, saving 98% of data analysis time, 61% of experiment planning time, 69% of assay execution time, compared to manual ELISAs
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  • Synthace offers higher dilution accuracy, resulting in better percentage recovery of samples quantified, and a 1.5-fold improvement in average interplate CV (%) compared to manual runs

Executive summary

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The enzyme-linked immunosorbent assay (ELISA) is one of the most commonly used immunoassays for quantifying antigens. Traditionally, the assay has been performed manually, which is strenuous, time-consuming, variable, and error-prone. To address these pain points and empower scientists to perform faster, more efficient, and more robust ELISAs, we have built an automated, end-to-end ELISA solution using Synthace, our cloud-based software platform.

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In this application note, we demonstrate how Synthace-driven ELISA combines three independent devices - the Hamilton Microlab® STAR liquid handler, the BioTek® 405 TS plate washer, and the BMG CLARIOstar® plate reader - into an integrated ELISA workcell. Synthace then orchestrates and tracks metadata generated during the experiment to perform instant data analysis using a four-parameter logistic fit.

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Synthace delivers a fully digitized ELISA experience, while providing higher dilution accuracy and a 1.5-fold improvement in average interplate CV (%) along with a 71% reduction in hands-on time, including a 98% reduction in data analysis time.

98%

Shorter data analysis time

71%

Shorter hands-on time

1.5-fold

Improvement in average interplate CV(%)

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