Technical Note: Enhancing RoboColumn® Workflows with Synthace and Te-Chrom™

Study summary
.

We compared miniaturized purification using two software platforms: Tecan’s Freedom EVOware® Te-Chrom™ Wizard and Synthace's cloud-based software.
.

We used both platforms to generate instructions for liquid handling robots for purification of IgG from human serum using MabSelect™ PrismA RoboColumns®.

Key findings
.

• Equivalence between Synthace and Freedom EVOware® generated instructions
.

• Significant time savings with Synthace's data structuring and visualization functionalities, blanks are easily specified and automatically used in data correction
.

• Enhanced flexibility with Synthace, e.g. specifying multiple buffers within individual chromatography stages

80%

Reduction in data processing, visualization and analysis time

50%

Saving in time spent adapting experimental design between runs

58%

Time saving in selecting, pooling, and normalizing samples

🔝