Rapid Automation and Analysis of a Competitive ELISA Kit with Synthace

Key findings
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  • Rapid adaptation of an automated sandwich ELISA protocol to a competitive format in 15 min using our Synthace software
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  • Synthace integrated three independent devices into a single automated workcell and provided instant data analysis and visualization, leading to immediate insights
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  • Low variability between technical replicates - average values for standards and samples from Synthace-automated liquid handling were 6.0% and 3.9%, respectively
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  • Excellent quantification efficiency with all samples made from Synthace-driven automation lying within the 80-120% percentage recovery range

Executive summary
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In this study, we demonstrate the rapid and flexible automation of a kit-based competitive ELISA protocol, from design through execution to data analysis, using our software Synthace. This work expands on our sandwich ELISA application note: here we rapidly adapted the previously-described sandwich ELISA protocol to the competitive ELISA format in only 15 minutes.
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Synthace orchestrated the execution of this protocol across three integrated devices (Hamilton® Microlab® STAR™ liquid handler, BioTek® 405™ TS plate washer, BMG LABTECH CLARIOstar® plate reader) and performed instant data analysis and visualization, leading to immediate insights.
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Synthace-driven automation achieved low variability results and excellent quantification accuracy compared to manual execution, thereby delivering a rapid, fully-digitalized, and flexible solution for maximizing the efficiency of competitive ELISAs and boosting overall productivity of scientific teams.

Video preview: multi-device integration with Synthace.
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