Construct Assembly for the Development of New Therapeutics with Ipsen

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Synthace sits very well at the beginning of our drug discovery process. It allows us to explore larger drug design space by simplifying planning and production of multiple molecule variants with combinatorial construct assembly. These throughput improvements help us deliver well tolerated and effective therapeutic solutions more rapidly to our patients. Karen Bunting, Director of Protein Sciences Ipsen

Accelerating the development of new therapeutics with flexible, automated construct assembly

Ipsen began working with Synthace in 2018. The Neuroscience group at Ipsen utilized Synthace’s software platform to empower its research into the discovery of new targeted secretion inhibitors (TSIs®), a new class of biopharmaceuticals.

Synthace was deployed to accelerate the generation of new molecules using the Type IIS construct assembly method, aiming to increase its flexibility, efficiency, and throughput, which would enable more rapid development of new therapeutics, advancing these towards the patient.

Key findings

With Synthace, Ipsen's Type IIS construct assemblies were:

5x quicker

Time needed to develop 24-48 constructs decreased from 30 to 6 days compared to manual assemblies

10x cheaper

Core DNA building blocks could be reused in multiple reactions, reducing the associated costs

Ipsen user testimonial*

*for the complete testimonial table, see the full case study

  • Manual construct assemblies

    Synthace-automated construct assemblies

  • Reproducibility

    Manual construct assemblies

    • There is potential for pipetting errors, especially in complex reaction setups
    • Method transfer between scientists may require detailed explanation which is often time-consuming and can still be misinterpreted
    • Any changes to sample concentrations or volumes require repeated calculations and extensive modification of the experimental protocols

    Synthace-automated construct assemblies

    • Multi-part construct assemblies and associated plate layouts for one pot reactions are reproducible because everything is tracked and recorded in Synthace
    • Methods are accessible and can be reproduced by multiple lab users and on different automated liquid handlers
    • Modifications to experimental workflows are straightforward to implement and the desired changes can be tested in silico
  • Time comparison

    Manual construct assemblies

    • Sophisticated constructs may require multiple cloning steps, increasing the number of liquid transfers involved, the potential for human error, and the linear time of the assembly reactions
    • Manual pipetting does not allow any walkaway time

    Synthace-automated construct assemblies

    • More constructs can be handled in the same linear time frame compared to the manual approach
    • Walkaway time is increased freeing up scientists for other activities