DOE for Assay Development

Why DOE assays with Synthace?

Developing high performing robust assays is a multi-factorial and challenging process. 
 
DOE is an ideal tool for developing assays with desired traits, but executing a complex DOE design on a liquid handler requires advanced automation programming skills.
 
For this reason, laboratories find it very difficult to implement DOE optimizations to assay protocols and processes.

 

Flexibility, allowing complex experimental design

Synthace removes all major barriers to entry for scientists wanting to use DOE to optimize their assays.

Synthace provides an interface that converts DOE designs into automation instructions for DOE-driven assay optimization. 

Convert

Synthace can convert DOE design information into executable instructions for liquid handlers

Trace

Synthace methods and runs are fully traceable for easy review and comparison

Merge

Synthace merges data acquisition and structuring from analytical devices for faster analysis

Pain points and current processes

  • Current practices

    With Synthace

  • Experiment inputs

    Current practices

    • Difficult programming creates a high barrier to entry for complex experiment design
    • Complex design can be time consuming to program and troubleshoot

    With Synthace

    • User-friendly codeless programming lowers the barrier to entry and allows more members to plan complex designs
    • Synthace's flexible setup creates liquid handling steps based on the user’s experimental design
  • Experiment planning

    Current practices

    • Rigid automation protocols make adapting experimental protocols slow and painful
    • User must be physically at device to plan and program workflows, limiting experiment development and slowing process

    With Synthace

    • Plan your experiment remotely, from anywhere
    • Flexibly change experimental parameters with Synthace's intuitive interface and dynamically reprogram the automation
  • Experiment execution

    Current practices

    • Complex liquid transfer steps must be calculated, resulting in less sophisticated experiments
    • Errors lead to repeat experiments costing time and resources

    With Synthace

    • Synthace performs all liquid transfer calculations, ensuring accuracy
    • Highly reproducible experiments cut down on failed runs
  • Data aggregation

    Current practices

    • Matching the data that a device outputs to your original design requires manual input involving multiple steps

    With Synthace

    • Synthace provides full traceability of samples from start to finish
    • DOE results, plate layouts, device instructions and reagents required all found on one page
  • Data analysis

    Current practices

    • No easy way to link with peripheral hardware

    With Synthace

    • Synthace exports your DOE output data into accessible formats
    • Synthace links to additional hardware, including plate readers
  • Experiment outputs

    Current practices

    • Difficult to compare data and methods with previous runs
    • Sharing automation instructions with other sites/devices means lengthy reprogramming

    With Synthace

    • Previous data sets stored in the cloud for easy access
    • Device agnostic protocols are easily shared between lab members from any site

Protocol walkthrough

Basic concepts

Translate human-readable actions into machine instructions

Simple building blocks...

Elements are a key concept of the Synthace platform, they form the visual user interface to Synthace’s planning engine and translate human readable actions into machine instructions. You can easily define how the experiment will be performed by modifying the element input parameters.

Using these building blocks, you can quickly create intricate workflows in Synthace by connecting elements together to specify the flow of physical materials and information.

...translated into complex robot instructions

You build the workflow, Synthace interprets your requirements and translates these into actionable robot instructions. Additionally, input volumes are automatically calculated and the labware needed is determined for you.

Define experiment

Easily define and modify your experiment

Flexibly plan and optimize your assay

Upload designs from Design Expert or JMP into Synthace to build DOE workflows

Adapt your protocols quickly and easily

Modify and change your DOE design and experiment parameters without having to reprogram your devices by simply uploading a new design

Speed up your analysis by removing manual file aggregation

Synthace’s Execution Details page contains all design and experiment files eliminating the need for manual structuring

Preview in silico

Preview your runs in detail at your desk

Synthace’s execution preview shows a layout of your device, and clearly shows where labware needs to be placed and the amount and location of your reagents.

The preview also shows every liquid handling action in order of execution with a useful step-by-step time estimate. This minimizes the chances of errors, and gives you an immediate estimate of walk-away time. Hovering over individual transfers will indicate the selected liquid handling policies.

Output

Data structuring and export

Once the experiment has completed, the user's data is securely sent back to the Synthace cloud. This data can then be exported in structured data tables in common formats. All data and methods can easily be compared with previous runs.

Introducing Synthace, the world’s most powerful software platform for performing biological experiments

​​Intelligent software for the dynamic use of laboratory automation

Sophisticated experiments made simple

FAQs

Synthace for DOE: assay development is available on:

  • Tecan's Freedom EVO®
  • Hamilton's Microlab® STAR and STARlet
  • Gilson's PIPETMAX®
  • FORMULATRIX®'s MANTIS®
  • SPT Labtech's dragonfly® discovery

Synthace provides a wide range of integration options for analytical instruments. We have direct integration with most multimode plate readers commonly used with assay optimization. If you’d like to enquire about specific processes for your lab, please drop us an email or use the “Request a demo” button above.

The Synthace planning engine parses the high-level inputs specified in your workflow, including the experimental design, selected labware and deck layout. The planning engine converts these instructions into low-level instructions such as the liquid handling steps and the exact labware positions on your predefined deck layout.

Yes, custom deck layouts can be chosen in Synthace. The user can define multiple deck configurations for the same liquid handler mode.

SynthaceHub automatically sends log and configuration files to the cloud for storage in your organization’s private data repository in Synthace from the liquid handler’s native software.

Although calendar integrations are not yet available in Synthace, the estimated run times as calculated by Synthace’s planning engine provide an accurate timing for you to ensure that you have booked enough time on your desired devices to execute your experiments.

Synthace will work out the changes necessary to implement your workflow. You simply need to upload your deck configuration and re-simulate — Synthace will use your new worktable layout to determine the necessary instructions.

Synthace comes with its own library of different liquid handling policies for all types of liquids. You can select different liquid handling policies for each of the liquid handling steps. Different samples can have different policies, and these policies can be linked to your liquid classes on certain liquid handling devices.

Yes, Synthace’s cherry picker is one example of that. Another example would be DOE runs with hard to change factors where we would need to run across two workflows.

Experimental results can be searched and accessed by everyone in your group/organization who has an account in Synthace. If you would like to discuss access control and data security in more detail, please drop us an email or use the “Request a demo” button above.

Trusted by

We were founded by a team of biologists in 2011 as a bio-process service provider, optimizing the existing bio-processes of our clients using a multi-factorial experimental approach. We used automated robotic equipment to run our high-dimensional, complex experiments.

Synthace has helped us improve our business and think outside the box, and its platform has allowed us to do many things we previously couldn’t” André Raposo Group Lead - Computer-Aided Biology (CAB) group at Oxford Biomedica
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