
Optimizing Construct Assembly with Synthace
Read the full reportKey findings
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Synthace improved the robustness and reproducibility of Design of Experiments (DOE) optimization of BsaI DNA-assembly protocols, ensuring >95% correct 7-part DNA assemblies
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The optimized experimental workflow was initially programmed for a Gilson PIPETMAX® liquid handler and easily transferred to a Hamilton Microlab® STAR, FORMULATRIX® MANTIS® and Tecan Freedom EVO® robots, achieving comparable robustness
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The automated Synthace approach reduced time spent per experiment from 29.5 hours to 8.5 hours, resulting in a 21 hour time saving; up to 63 hours were saved across the whole study
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Overall, Synthace achieved a 74% time saving in experimental planning, design and execution
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This means large efficiency savings in FTE time and a 22% reduction in tip usage
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Discover how Synthace-driven DOE optimization of 7-part DNA assembly achieved:
>95%
Correct constructs
74%
Time saving
22%
Reduction in tip usage