Optimizing Construct Assembly with Synthace

Key findings
.

• Synthace improved the robustness and reproducibility of Design of Experiments (DOE) optimization of BsaI DNA-assembly protocols, ensuring >95% correct 7-part DNA assemblies
  .

• The optimized experimental workflow was initially programmed for a Gilson PIPETMAX® liquid handler and easily transferred to a Hamilton Microlab® STAR, FORMULATRIX® MANTIS® and Tecan Freedom EVO® robots, achieving comparable robustness
  .

• The automated Synthace approach reduced time spent per experiment from 29.5 hours to 8.5 hours, resulting in a 21 hour time saving; up to 63 hours were saved across the whole study
  .

• Overall, Synthace achieved a 74% time saving in experimental planning, design and execution
  .

• This means large efficiency savings in FTE time and a 22% reduction in tip usage
  .