Miniaturized Gradient Elution with Synthace

Study summary
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  • We used Synthace to plan, execute and analyse data from miniature gradient elution experiments using 200 µL RoboColumns® on a Tecan Freedom EVO® liquid handler
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  • We studied how liquid handling behaviour influences gradient generation and evaluated the impact of pH, gradient slope, and residence time on the separation of a mixture of four proteins
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  • Selected fractions were automatically cherry picked and analysed for purity using a LabChip® GXII Touch™ device. Resulting purity data was automatically aligned to chromatography data
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Key findings
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  • We identify a set of conditions to separate four proteins contained in a reference solution
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  • Synthace enables flexible RoboColumn workflows, allowing users to define gradient conditions by easily specifying buffer types and gradient slopes. All intermediate buffers are prepared on the fly
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  • Synthace automatically performs data alignment, normalization and visualization, resulting in immediate insights. Structured data can be downloaded for analysis with external tools

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