Device-Agnostic Protocols Using Synthace: a qPCR Example

Key findings

• Synthace enables execution of sophisticated automation protocols and their seamless transfer between different liquid handlers, which has not been shown before
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• Using Synthace, we executed the same automated qPCR protocol on three different liquid handlers (Gilson PIPETMAX®, Hamilton Microlab® STAR™, Tecan Freedom EVO® 100) and achieved consistent results:

• • Standard curves with correct primer efficiencies (100-103%, R² ≥ 0.99)
  • Comparable Cq values
  • CVs ≤ 1% across technical replicates for different DNA concentrations
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• Synthace overcomes the challenges of laboratory automation and facilitates its adoption in biological R&D