
Device-Agnostic Protocols Using Synthace: a qPCR Example
Read the full reportKey findings
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Synthace enables execution of sophisticated automation protocols and their seamless transfer between different liquid handlers, which has not been shown before
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Using Synthace, we executed the same automated qPCR protocol on three different liquid handlers (Gilson PIPETMAX®, Hamilton Microlab® STAR™, Tecan Freedom EVO® 100) and achieved consistent results:
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- Standard curves with correct primer efficiencies (100-103%, R² ≥ 0.99)
- Comparable Cq values
- CVs ≤ 1% across technical replicates for different DNA concentrations
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Synthace overcomes the challenges of laboratory automation and facilitates its adoption in biological R&D
